Expertise for label-free technique for hit finding and hit/lead characterization.
Surface-plasmon resonance (SPR) is the method of choice for estimating the kinetics of a binary interaction (involving proteins, peptides, nucleic acids, small molecules). Identifying the association and dissociation rates (kon; koff) of a drug candidate is critical for monitoring the residence time and maximizing target engagement. This highly sensitive technology can support drug discovery from hit to lead stage. SPR is powerful for deciphering any types of interaction parameters such as highly potent and/or slow dissociating compounds
Our highly dedicated team has built its experience on a variety of challenging projects, always with the view of offering our clients the strategy best suited to their needs.
Areas of expertise include:
- Nucleic acids: DNA; RNA (miRNA: post-transcriptional regulation)
- Immuno-modulators
- Antioxidant inflammation modulators
- Protein degradation (PROTAC and molecular glues)
- Transmembrane receptors & GPCR
- Nuclear receptors, lectin, transcriptional factors
- Antibodies, Fabs and ScFv fragments
Assay formats encompasse:
- Screening: binding level screening at 1 & 3 analyte concentration (internal fragment library available or customer library up to 4K compounds)
- Direct binding: MCK and SCK experiment (kon, koff, KD, residence time)
- Competition assay
- Characterization of ternary complexes: cooperativity
- Epitope binning
Novalix also offers orthogonal biophysical methods, such as Microscale Thermophoresis (MST), Isothermal Titration Calorimetry (ITC), SEC-UV Native Mass Spectrometry (nMS), Differential Scanning Fluorometry (DSF/nDSF), Dynamic Light Scattering (DLS) and Nuclear Magnetic Resonance (NMR).
Protac ligand binding to 2 different domains of BRD4 protein and to CEREBLON E3 ligase.
Explore how SPR can accelerate your hit finding and hit/lead characterization — contact us to learn more.