Crystallographic grade recombinant protein, suitable for biophysical techniques and biochemical assays, can be provided off-the-shelf or by customized production.
- in silico sequence analysis and design of multiple constructs to aid solubility and crystallization
- Prokaryotic (E. coli) and eukaryotic (insect cells and mammalian cells) production systems with codon-optimized genes
- Quality control using mass spectrometry, nano differential scanning fluorimetry (NanoDSF), Size-Exclusion Chromatography with Multi-Angle Light Scattering (SEC-MALS)
- Isotopic labeling
Novalix has built up expertise in difficult systems, such as nuclear hormone receptors (NHR), and has developed special protocols to produce membrane proteins, such as GPCRs.