At the last Promega Innovation Day in Leuven in May, Viviana Gatta from UCB Biopharma presented a team work on Nanoclick. The authors are Viviana Gatta, Martine Geraerts (UCB Biopharma), Noemie Willain and Davide Proverbio (both from Novalix).
Abstract of the presentation
Cytosolic bioavailability is a strong limiting factor for the development and delivery of new therapeutics active intracellularly. In fact, the majority of molecules gets internalized and redirected to lysosomes for degradation. Determining endosomal escape and cytosolic presence of potential drug candidates at early stage of the screening cascade can significantly impact the drug discovery process.
To answer this need, Nanoclick has been described as high-throughput and target agnostic assay which combines click chemistry and Nano-Bret signal for the detection of cell-penetrating peptides. We explored the potential of the assay to assess internalization of molecules mediated by specific protein of interest.
We will describe the optimization of live and digitonin conditions to enable determination of endosomal escape as well as the development of a target deficient line to confirm molecules specificity. This flexible setup can be adapted to different kind of therapeutics and targets, enabling the use of Nanoclick as tool for the discovery of target-mediated internalization.
Congratulations to Viviana Gatta and her team for exploring the potential of the Nanoclick essay to assess receptor mediated internalization (NanoClick: A High Throughput, Target-Agnostic Peptide Cell Permeability Assay | ACS Chemical Biology).
Insourcing, an innovative collaborative model
Since 2016, Novalix has worked in close partnership with UCB, a Belgium-based global biopharmaceutical company focused on the discovery and development of innovative medicines and solutions to transform the lives of people living with severe diseases of the immune system or of the central nervous system.
This collaborative model, named Insourcing, relies on the proximity created by the client’s and NovAliX’ teams both operating on the client’s site. This proximity allows smooth communication, faster turnaround time and improves cost efficiency, thanks to the perfect integration of Novalix team into the customer’s workflow. Insourcing also enables to improve operational efficiency by incorporating best lab practices, and to maintain highly qualified research capabilities locally.
