Over the past few years, Cryo-EM has been developed as a complementary tool to protein X-ray crystallography to solve difficult protein structures, such as multi-protein complexes, membrane proteins and antibodies, and is now used to support structure-based drug design projects.
In 2016, NovAliX and Thermo Fisher Scientific established a partnership and created a joint cryo-EM facility at NovAliX. Since then, a diverse range of projects have been successfully completed. The laboratory is equipped with a Glacios microscope, with a Falcon 3 detector, phase plate and micro-ED module. Titan Krios microscopes can be accessed as needed through academic partners and Thermo Fisher Scientific.
An efficient workflow supporting pharmaceutical R&D needs and spanning protein production to the delivery of high-resolution structures has been developed over time. This service, performed by experienced scientists with complementary skills, is divided into three packages:
- EM Essential: Customized solutions meeting client needs, from negative stain studies to high resolution cryo-EM structures
- Starter Kit and Explore : Packages include additional theoretical and practical education on cryo-EM.
|EM Essential||Starter Kit||Explore|
|Description||Customized solutions to meet client needs||Essential theoretical and practical introduction to EM||Insight into driving cryo-EM to high resolution|
|Activities||Fee-for-service or FTE-based projects||Observe sample preparation and performance of EM*||Observe your own sample under EM (negative stain / cryo-EM) and understand sample quality requirements for cryo-EM 3D reconstruction|
|Protein production||Observe your own sample under EM (negative stain / cryo-EM)||Opportunity for insight into vitrification and hands-on experience at our premises|
|Negative staining||Sample quality assessment for cryo-EM 3D reconstruction||Observe cryo-EM workflow performed on your own sample (aiming for high-resolution 3D reconstruction)|
|2D class average||*Optional|
If the sample has the required quality, up to cryo-EM 2D classification
|Initial 3D model||Learn cryo-EM basics||Learn cryo-EM theory (via preliminary learning material)|
|Cryo-EM 3D high resolution structure||2 days at our premises|