Biophysical Characterization

Broad panel of techniques for studying interactions

Our biophysical technologies allow us to select the best technique for measuring binding properties and characterizing interactions between small molecules, nucleic acids, lipids, sugars, peptides and proteins.

We can obtain diverse data including: KD, stoichiometry, reversibility of binding, competitive binding, kon, koff and thermodynamic parameters.

  • Mass Spectrometry: a versatile tool for drug discovery
    We have extensive experience in the use of native mass spectrometry and hydrogen-deuterium exchange mass spectrometry (HDX-MS) for protein characterization and interaction studies.
    Peptide mapping | covalent binding | Molecular weight | Affinity | Competition | Stoichiometry
  • Surface Plasmon Resonance (SPR): Kinetic parameters
    Our SPR experts can develop new methods for challenging targets or can efficiently transfer existing methods for routine analysis. Equilibrium constants, kinetics and screening are measured using a Biacore T200.
    Affinity | Stoichiometry | Kinetics: Kon & Koff | Competition | Cooperativity | Reversibility
  • Isothermal titration calorimetry (ITC): Thermodynamic parameters
    ITC is commonly used as a secondary or orthogonal technique to provide a real-time label-free direct measurement of binding in solution.
    Affinity | Stoichiometry | ∆G, ∆H and ∆S | Kinetics | Cooperativity
  • NMR: Gold standard for characterization
    Fragment screening, protein characterization and binding experiments, as well as quality control, are performed using 400 MHz to 800 MHz state-of-the-art instruments, and can include cryoprobe technology.
    1D/2D/3D NMR | qNMR | Interaction (Saturation transfer difference (STD), WaterLOGSY…) | HSQC mapping | Assignment | DOSY

We also offer micro scale thermophoresis (MST) and differential scanning fluorimetry (DSF). Dynamic light scattering (DSL), which is used to measure the critical aggregation concentration (CAC) of compounds or proteins, is highly recommended prior to any study.

Do you want to learn more?

Consult our experts regarding the optimal combination of services.

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